Composite

Part:BBa_K2959001:Design

Designed by: Alejandro Aguirre Hernndez   Group: iGEM19_Tec-Chihuahua   (2019-08-26)


Expressible LL 37 with Arabinose Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

This part uses the araC-Pbad - Arabinose inducible regulatory promoter/repressor unit (K808000), designed by iGEM12_TU_Darmstadt. The promoter is repressed by AraC (coded in the part) and induced by L-arabinose. This creates a controlled expression system with low background activity. iGEM12_Trieste optimized the sequence of LL 37 for E. coli expression.


Source

This part was constructed using parts previously added by other teams to the Registry: pBad-rbs-LL37 (BBa_K1230007) and a doulbe terminator (BBa_B0015)

References